Glycogen Metabolic Genes Are Involved in Trehalose-6-Phosphate Synthase-Mediated Regulation of Pathogenicity by the Rice Blast Fungus Magnaporthe oryzae
نویسندگان
چکیده
The filamentous fungus Magnaporthe oryzae is the causal agent of rice blast disease. Here we show that glycogen metabolic genes play an important role in plant infection by M. oryzae. Targeted deletion of AGL1 and GPH1, which encode amyloglucosidase and glycogen phosphorylase, respectively, prevented mobilisation of glycogen stores during appressorium development and caused a significant reduction in the ability of M. oryzae to cause rice blast disease. By contrast, targeted mutation of GSN1, which encodes glycogen synthase, significantly reduced the synthesis of intracellular glycogen, but had no effect on fungal pathogenicity. We found that loss of AGL1 and GPH1 led to a reduction in expression of TPS1 and TPS3, which encode components of the trehalose-6-phosphate synthase complex, that acts as a genetic switch in M. oryzae. Tps1 responds to glucose-6-phosphate levels and the balance of NADP/NADPH to regulate virulence-associated gene expression, in association with Nmr transcriptional inhibitors. We show that deletion of the NMR3 transcriptional inhibitor gene partially restores virulence to a Δagl1Δgph1 mutant, suggesting that glycogen metabolic genes are necessary for operation of the NADPH-dependent genetic switch in M. oryzae.
منابع مشابه
The Sugar Sensor, Trehalose-6-Phosphate Synthase (Tps1), Regulates Primary and Secondary Metabolism during Infection by the Rice Blast Fungus: Will Magnaporthe oryzaeâ•Žs â•œSweet Toothâ•š become Its â•œAchillesâ•Ž Heelâ•š?
The Sugar Sensor, Trehalose-6-Phosphate Synthase (Tps1), Regulates Primary and Secondary Metabolism during Infection by the Rice Blast Fungus: Will Magnaporthe oryzae's " Sweet Tooth " become Its " Achilles' Heel " ?" (2011). Fungal Molecular Plant-Microbe Interactions. Paper 6. disparate cellular activities? To address this question, this review discusses our current understanding of tre-halos...
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Magnaporthe oryzae (M. oryzae) is a fungal pathogen and the causal agent of rice blast disease. Previous lipidomics analysis of M. oryzae demonstrated that trehalose, a carbohydrate common to various fungi and algae, is thought to be involved in the possible conversion of glycogen into triacylglycerides for energy, an important step in the pathogenesis of M. oryzae. A key enzyme responsible for...
متن کاملThe Sugar Sensor, Trehalose-6-Phosphate Synthase (Tps1), Regulates Primary and Secondary Metabolism during Infection by the Rice Blast Fungus: Will <i>Magnaporthe oryzae</i>’s “Sweet Tooth” become Its “Achilles’ Heel”?
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Regulation of Cellular Diacylglycerol through Lipid Phosphate Phosphatases Is Required for Pathogenesis of the Rice Blast Fungus, Magnaporthe oryzae
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